Original Research

A Mouse Model for Chronic Cerebral Hypoperfusion-induced Cognitive Dysfunction

Jun Li , Zhi-Yi Zuo
From Department of Anesthesiology, University of Virginia
J Anesth Perioper Med 2017; 4(2): 60- 66 . Published on Mar 5, 2017 . doi:10.24015/JAPM.2017.0015
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Abstract

Background: Chronic cerebral hypoperfusion potentially contributes to the initiation and progression of vascular dementia (VD). Bilateral common carotid artery occlusion (two-vessel occlusion) is the most commonly used animal model to replicate this pathological condition but with high mortality and severe histological cerebral damage. Unilateral common carotid artery occlusion (one-vessel occlusion) was introduced to simulate clinical conditions. Our study was designed to further characterize this model.

Methods: In this study, eight-week old CD-1 mice were subjected to left common carotid artery occlusion (LCCAO). Two weeks after the occlusion, their learning and memory were assessed by Barnes maze and fear conditioning. Histo-morphological changes were evaluated by Hematoxylin-Eosin staining. Neuronal and axonal degenerative changes were examined by amino-cupric sliver staining.

Results: LCCAO increased the time to find the target box in the Barnes maze test during the 4-day training sessions and one day after the training sessions compared with sham group mice. There was no difference in context-related or tone-related freezing behavior between these two groups. No significant histological neuronal cell damage or degeneration was observed in brain sections of hippocampus and corpus callosum in these two groups.

Conclusions: Our results suggest that LCCAO can be used to mimic the vascular dementia.

 

 

Citation:  Jun Li, Zhi-Yi Zuo. A mouse model for chronic cerebral hypoperfusion-induced cognitive dysfunction. J Anesth Perioper Med 2017; 4: 60-66. doi:10.24015/JAPM.2017.0015

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