Original Research

Astragalus Pretreatment but Not Posttreatment Reduces Hemorrhagic Shock-Induced Intestinal Injury in a Rat Model

Yu Gu , Jiaxin Chen , Nan Cheng , Dezhao Liu , Mian Ge , Chaojin Chen , Ziqing Hei
Department of Anesthesiology, the Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
J Anesth Perioper Med 2017; 4(6): 237- 242 . Published on Nov 28, 2017 . doi:10.24015/ JAPM.2017.0192
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Background: To observe the expression of inducible nitric oxide synthase (iNOS) and endothelin (ET) in small intestinal mucosa of rats which experienced hemorrhagic shock/reperfusion (HS/R) injury,  and further explores the effect of astragalus in HS/R process. 
Methods: Thirty-two male SD rats were randomly divided into SHAM group, HS group,  HS+Pre-AS group, and HS+Post-AS group. Except for the rats in the SHAM group, the rest rats were experienced severe uncontrolled hemorrhagic shock and recovery treatment, the rats in HS+Pre-AS group were intravenous injected astragalus 15 g/kg/d for 7days before the experiment,  the rats in HS+Post-AS group were intravenous injected astragalus 20 g/kg after ischemia.   Then rats were killed and the small intestinal mucosa was obtained for H&E staining to observe the pathological changes of the intestine, blood was collected to detected serum MDA, SOD activity, NO levels, and the expressions of iNOS and ET in the intestinal mucosa were detected by western blot. 
Results: There was no intestinal mucosa injury in SHAM group, the most serious intestinal mucosa injury appeared in HS group, astragalus had a protective effect on small intestinal mucosa in ischemia-reperfusion models. Compared with the SHAM group, the Chiu’s scores in HS group were the largest, accompanied by an increase of serum MDA and decrease of serum SOD activity.   Pretreatment with astragalus significantly alleviated intestinal pathological injury, decreased MDA and enhanced SOD activity, but not post-treatment. Meanwhile, the expressions of iNOS and ET-1 increased significantly in HS group and HS+Post-AS group. Compared with HS group, the expression of iNOS and ET-1 in the HS+Pre-AS group were significantly alleviated.   
Conclusion: Astragalus pretreatment might protect the intestine from uncontrolled HS/R injury,  which might be associated with the reduction of the iNOS and ET-1 expression. However,  post-treatment with astragalus had no the same protective effect. (Funded by the National Natural Science Foundation of China, Science and Technology Project of Guangdong Province and Guangzhou Science and Technology Plan, China.) 
Citation: Yu Gu, Jiaxin Chen, Nan Cheng, Dezhao Liu, Mian Ge, Chaojin Chen, et al. Astragalus Pretreatment but Not Posttreatment Reduces Hemorrhagic Shock-Induced Intestinal Injury in a Rat Model. J Anesth Perioper Med 2017; 4 : 237-42. doi: 10.24015/ JAPM.2017.0192
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