Original Research

Altered Expression of 5-HT Receptor Subtypes in the Spinal Cord After Cardiac Ischemia-Reperfusion Injury

Zhi-Xiao Li , Zhi-Gang He , Shun-Yuan Li , Hong-Bing Xiang
Department of Anesthesiology and Pain Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China, Department of Anesthesiology, The First Affiliated Quanzhou Hospital of Fujian Medical University, Quanzhou, China
J Anesth Perioper Med 2019; 6(x): x- xx . Published on Jan 5, 2019 . doi:10.24015/JAPM.2019.0007
Abstract
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Abstract

Background: Spinal cord plays an important role in the development and progression of cardiac ischemia-reperfusion injury (CIRI).Numerous studies have been performed to identify the specific role of cardiac 5-hydroxytryptamine (5-HT) in cardiomyocyte damage, whereas reports about the potential role of spinal 5-HT during cardiac ischemia are rare.  We investigated the involvement of 5-HT receptor subtypes in the spinal cord after CIRI.

Methods: Male adult SD rats (250 ~ 300 g) were randomly divided into two groups: Control group (Sham, n = 10) and Model group (30 min ischemia followed by 2 h reperfusion, n = 10). The animals of Control group had the same operation (thoracotomy) as the Model group but without left anterior descending coronary artery ligation. By using real-time quantitative polymerase chain reaction (RT-qPCR), we drew the expression profiles of 5-HT receptor subtypes in the upper thoracic segment of the spinal cord after CIRI.

Results: We found that there was a significant decrease of 5-HT1A, 5-HT1B, 5-HT1D, 5-HT3C, 5-HT5B, and 5-HT6 expression after CIRI, indicating that the specific role of 5-HT receptor subtypes in the spinal cord after CIRI.

Conclusion: Our results will be beneficial for understanding the serotonergic drug targets to decrease CIRI in future preclinical and clinical studies. (Funded by the the National Natural Science Foundation of China, National Natural Science Foundation of Hubei Province, and Medical innovation project in Fujian Province, all in China.)

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